Preliminary Phytochemical Screening and Antibacterial Activity of the Crude Methanol Leaf Extract of Diospyros Mespiliformis Hochst (EBENACEAE)

Amos Tabitha Buba, Dr. Muhammad A. Tijjani, Dr. Tagwi A. Balami (DVM)
International Journal of Analytical, Experimental and Finite Element Analysis
Volume 8: Issue 4, December 2021, pp 113-116


Author's Information

Amos Tabitha Buba1 

Corresponding Author
1University of Maiduguri/ Pure and Applied Chemistry, Maiduguri, Nigeria

Dr. Muhammad A. Tijjani2, Dr. Tagwi A. Balami (DVM)2

2University of Maiduguri/ Pure and Applied Chemistry, Maiduguri, Nigeria


Short Paper -- Peer Reviewed
Published online – 31 December 2021

Open Access article under Creative Commons License

Cite this article – Amos Tabitha Buba, Dr. Muhammad A. Tijjani, Dr. Tagwi A. Balami (DVM), “Preliminary Phytochemical Screening and Antibacterial Activity of the Crude Methanol Leaf Extract of Diospyros Mespiliformis Hochst (EBENACEAE)”, International Journal of Analytical, Experimental and Finite Element Analysis, RAME Publishers, vol. 8, issue 4, pp. 113-116, 2021.
https://doi.org/10.26706/ijaefea.4.8.20211201


Abstract:-
Diospyros mespiliformis, is an evergreen tree found mostly in the savannas of Africa. The leaves have wide applications in traditional medicine which includes whooping cough, wound dressing, pneumonia, syphilis, arthritis and skin infections. The preliminary phytochemical screening showed the presence of cardiac glycosides, terpenoids, alkaloids, saponins, tannins, anthraquinones and flavonoids. The in vitro antibacterial study of crude methanol leaf extract (CME) was analysed using disc diffusion method (DDM), with two-gram positive (Staphylococcus aureus and Streptococcus pneumonia) and two-gram negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The standard antibiotic disc used was gentamicin (5µg/disc). The CME showed highest activity against Streptococcus pneumonia (12.5±1.12mm) at 150mg/ml and the lowest activity was seen against Staphylococcus aureus (11.5±1.11mm) at 200mg/ml as compared to 31.0±0.82mm and 41.0±0.82mm of the control (standard drug) respectively. Pseudomonas aeruginosa showed resistance against the CME at all concentrations including the standard drug.
Index Terms:-
Diospyros Mespiliformis, Phytochemical, Antibacterial, Crude methanol leaf extract.
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