Preliminary Phytochemical Screening and Antibacterial Activity of the Crude Methanol Leaf Extract of Diospyros Mespiliformis Hochst (EBENACEAE)
Amos Tabitha Buba, Dr. Muhammad A. Tijjani, Dr. Tagwi A. Balami (DVM)
International Journal of Analytical, Experimental and Finite Element Analysis
Volume 8: Issue 4, December 2021, pp 113-116
Amos Tabitha Buba1
1University of Maiduguri/ Pure and Applied Chemistry, Maiduguri, Nigeria
Dr. Muhammad A. Tijjani2, Dr. Tagwi A. Balami (DVM)2
2University of Maiduguri/ Pure and Applied Chemistry, Maiduguri, Nigeria
Abstract:-Diospyros mespiliformis, is an evergreen tree found mostly in the savannas of Africa. The leaves have wide applications in traditional medicine which includes whooping cough, wound dressing, pneumonia, syphilis, arthritis and skin infections. The preliminary phytochemical screening showed the presence of cardiac glycosides, terpenoids, alkaloids, saponins, tannins, anthraquinones and flavonoids. The in vitro antibacterial study of crude methanol leaf extract (CME) was analysed using disc diffusion method (DDM), with two-gram positive (Staphylococcus aureus and Streptococcus pneumonia) and two-gram negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The standard antibiotic disc used was gentamicin (5µg/disc). The CME showed highest activity against Streptococcus pneumonia (12.5±1.12mm) at 150mg/ml and the lowest activity was seen against Staphylococcus aureus (11.5±1.11mm) at 200mg/ml as compared to 31.0±0.82mm and 41.0±0.82mm of the control (standard drug) respectively. Pseudomonas aeruginosa showed resistance against the CME at all concentrations including the standard drug.
Index Terms:-Diospyros Mespiliformis, Phytochemical, Antibacterial, Crude methanol leaf extract.
- Burkill, H., M. “Useful plants of west Africa”, Royal botanical gardens, kew England, vol. 3, 1985, pp. 164-205.
- Dalziel, J., M., “The useful plants of west tropical Africa”, 2nd ed., vol. 2. Crown agents for the colonies London 1985, pp. 612.
- Irvine, J., R. Woody plants of Ghana. Oxford University press London, 1961, pp.51.
- Hochst, A., D. Useful tropical plants. Retrieved, 2018, pp. 20.
- Hassan, M., M., Oyewale, A., O., Amupitan, J., O., Abduallahi, M., S. and Okonkwo, E., M., “Preliminary Phytochemical and antibacterial investigation of crude extracts of the root bark of Detarium microcarpum”, Journal of Chemical Society of Nigeria, 2004, pp. 26-29.
- Sofowora, A. “Screening plants for bioactive agents”, Medicinal plants and traditional medicine in Africa. 2nd ed., 1993, pp. 134-156.
- Maydell, H. Trees and shrubs of the sahel, “Their characteristics and uses”, Verlag Josef Margraf Scientific Books. Welkershelm, Germany, 1990.
- Watt, J., M. and Brandwijk, M., G. “The Medicinal and Poisonous Plants of Southern and Eastern Africa”. 2nd ed., Livingstone Edinburg. 1962, pp. 369.
To view full paper, Download here